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Tools Collection 2024

The JCB editorial team is pleased to present a collection of recent Tools articles that most engaged our readers. These papers offer advances in methods and resources that open new capabilities and avenues for investigation, each with proof-of-concept demonstrations that reveal novel biology. This Tools collection encompasses the full breadth of fields published by JCB, including biomolecular condensates, single-molecule imaging, mRNA distribution across the nervous system, and novel mouse reporter strains.

We hope you enjoy this collection and we welcome your feedback on X/Twitter at @JCellBiol. Sign up to receive notifications about future special collections here.

Image © 2023 Jones et al.

Special Collection Image
Tools
hGRAD: Versatile “one-fits-all” rapid depletion
Benjamin Arnold; Ricarda J. Riegger; Ellen Kazumi Okuda; Irena Slišković; Mario Keller; Cem Bakisoglu; François McNicoll; Kathi Zarnack; Michaela Müller-McNicoll
https://doi.org/10.1083/jcb.202304030
Tools
Live visualization of the murine basement membrane
Rebecca A. Jones; Brandon Trejo; Parijat Sil; Katherine A. Little; H. Amalia Pasolli; Bradley Joyce; Eszter Posfai; Danelle Devenport
https://doi.org/10.1083/jcb.202309074
Tools
MCS-DETECT reveals dual control of riboMERCs
Ben Cardoen; Kurt R. Vandevoorde; Guang Gao; Milene Ortiz-Silva; Parsa Alan; William Liu; Ellie Tiliakou; A. Wayne Vogl; Ghassan Hamarneh; Ivan R. Nabi
https://doi.org/10.1083/jcb.202206109
Tools
Quantitative inventory of yeast actin cytoskeleton
Sofia Gonzalez Rodriguez; Alison C.E. Wirshing; Anya L. Goodman; Bruce L. Goode
https://doi.org/10.1083/jcb.202306036
Tools
A novel probe reveals the initial steps of lysophagy
Takayuki Shima; Monami Ogura; Ruriko Matsuda; Shuhei Nakamura; Natsuko Jin; Tamotsu Yoshimori; Akiko Kuma
https://doi.org/10.1083/jcb.202204048
Tools
PIKfyve regulation in phagosome maturation
James H. Vines; Hannes Maib; Catherine M. Buckley; Aurelie Gueho; Zhou Zhu; Thierry Soldati; David H. Murray; Jason S. King
https://doi.org/10.1083/jcb.202209077
Tools
Ultrafast single fluorescent-molecule imaging
Takahiro K. Fujiwara; Shinji Takeuchi; Ziya Kalay; Yosuke Nagai; Taka A. Tsunoyama; Thomas Kalkbrenner; Kokoro Iwasawa; Ken P. Ritchie; Kenichi G.N. Suzuki; Akihiro Kusumi
https://doi.org/10.1083/jcb.202110160
Tools
Systematic analysis of mRNA/protein discordance
Joshua S. Titlow; Maria Kiourlappou; Ana Palanca; Jeffrey Y. Lee; Dalia S. Gala; Darragh Ennis; Joyce J.S. Yu; Florence L. Young; David Miguel Susano Pinto; Sam Garforth; Helena S. Francis; Finn Strivens; Hugh Mulvey; Alex Dallman-Porter; Staci Thornton; Diana Arman; Marissa J. Millard; Aino I. Järvelin; Mary Kay Thompson; Martin Sargent; Ilias Kounatidis; Richard M. Parton; Stephen Taylor; Ilan Davis
https://doi.org/10.1083/jcb.202205129
Tools
Mammalian cellular fractionation for proteomics
Yael Udi; Wenzhu Zhang; Milana E. Stein; Inna Ricardo-Lax; Hilda A. Pasolli; Brian T. Chait; Michael P. Rout
https://doi.org/10.1083/jcb.202209062
Tools
AI to track mitotic spindle movements in 3D
David Dang; Christoforos Efstathiou; Dijue Sun; Haoran Yue; Nishanth R. Sastry; Viji M. Draviam
https://doi.org/10.1083/jcb.202111094
Tools
A live-cell marker for stable microtubules
Klara I. Jansen; Malina K. Iwanski; Mithila Burute; Lukas C. Kapitein
https://doi.org/10.1083/jcb.202106105

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