Figure S3. Annexins within EVs are... | Rockefeller University Press

Figure S3.

$Annexins within EVs are shifted in apparent molecular weight. (A) Immunoblots show enrichment of indicated EV markers relative to cell lysate after treatment of C2C12 myotubes with 200 ng/ml SLO. F1, F2, F3, and F4 refer to buoyant fractions of a sucrose step gradient of the conditioned medium 100k × g pellet fraction, moving from low to high density. (B and C) (B) Immunoblot and (C) luminescence analysis of the 10k × g pellet fraction (10k), the 100k × g pellet fraction (100k), and the remaining soluble supernatant (Sol.) after serial centrifugation of conditioned media from ANXA2-Nluc cells treated with 200 ng/ml SLO. For each fraction, Nluc luminescence (lumin.) was measured with or without membrane-impermeable Nluc inhibitor (Inh.). Source data are available for this figure: SourceData FS3.$

Annexins within EVs are shifted in apparent molecular weight. (A) Immunoblots show enrichment of indicated EV markers relative to cell lysate after treatment of C2C12 myotubes with 200 ng/ml SLO. F1, F2, F3, and F4 refer to buoyant fractions of a sucrose step gradient of the conditioned medium 100k × g pellet fraction, moving from low to high density. (B and C) (B) Immunoblot and (C) luminescence analysis of the 10k × g pellet fraction (10k), the 100k × g pellet fraction (100k), and the remaining soluble supernatant (Sol.) after serial centrifugation of conditioned media from ANXA2-Nluc cells treated with 200 ng/ml SLO. For each fraction, Nluc luminescence (lumin.) was measured with or without membrane-impermeable Nluc inhibitor (Inh.). Source data are available for this figure: SourceData FS3.

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