Annexin-containing EVs are distinct from exosomes. (A) Immunoblots show enrichment of exosome proteins from the conditioned medium 100k × g pellet (pellet input) after immunoprecipitation with protein G beads when anti-CD63 antibody (Ab) is added to the binding reaction (FT—binding reaction flow through; Elu—binding reaction elution). (B) Immunoblots show the distribution of EV markers across a sucrose step gradient of the conditioned medium 100k × g pellet fraction. Samples were taken from low density (F2-Fraction #2) to high density (F4-Fraction #4). Source data are available for this figure: SourceData FS1.