Figure 3. Annexins within EVs are... | Rockefeller University Press

Figure 3.

$Annexins within EVs are shifted in apparent molecular weight. (A) Immunoblots of gradient fractions show enrichment of indicated EV markers after 200 ng/ml SLO treatment, with or without 1 mM Ca2+ in the media. F2, F3, and F4 refer to buoyant fractions of a sucrose step gradient of the conditioned medium 100k × g pellet fraction. (B) Immunoblot analysis of cell lysates after treatment with indicated combinations of 1 mM Ca2+ and 200 ng/ml SLO is shown. (C) Immunoblots show separation of indicated EV markers after 200 ng/ml SLO treatment. F2–F15 refer to fractions #2–15 of an iodixanol gradient of the conditioned medium 100k × g pellet fraction, moving from low to high density. Source data are available for this figure: SourceData F3.$

Annexins within EVs are shifted in apparent molecular weight. (A) Immunoblots of gradient fractions show enrichment of indicated EV markers after 200 ng/ml SLO treatment, with or without 1 mM Ca²⁺ in the media. F2, F3, and F4 refer to buoyant fractions of a sucrose step gradient of the conditioned medium 100k × g pellet fraction. (B) Immunoblot analysis of cell lysates after treatment with indicated combinations of 1 mM Ca²⁺ and 200 ng/ml SLO is shown. (C) Immunoblots show separation of indicated EV markers after 200 ng/ml SLO treatment. F2–F15 refer to fractions #2–15 of an iodixanol gradient of the conditioned medium 100k × g pellet fraction, moving from low to high density. Source data are available for this figure: SourceData F3.

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