Data associated with Fig. 1 . (A) Western blot analysis of cell lysates from WT and sei1Δ cells endogenously expressing Pex30-GFP. Anti-GFP monoclonal antibody was used to detect Pex30 protein levels, and anti-Porin1 monoclonal antibody was used to detect porin levels as a control. (B) Quantification of protein levels from A. Bars show the mean from three replicates and SEM. One-way ANOVA and Dunnett’s multiple comparison test were used to compare protein levels. (C) WF images of sei1opi1Δ cells endogenously expressing Pex30-2xmCherry in logarithmic phase. White arrowheads show Pex30 accumulation. Bar = 4 μm. (D) WF images of WT and sei1Δ cells endogenously expressing Pex30-2xmCherry and Rtn1-GFP in logarithmic phase. Bar = 4 μm. (E) WF image of sei1Δ cells endogenously expressing Opi1-mCherry and Sec63-GFP on a plasmid as an ER marker in logarithmic phase. Bar = 4 μm. (F) Quantification of the localization of Opi1-mCherry puncta from E. (G) Phospholipid measurements of indicated strains (n = 3) by LC-HRMS. Distribution of total amounts of annotated PA species (*P < 0.05, **P < 0.01, and ***P < 0.001). Source data are available for this figure: SourceData FS1.. WF, widefield images; LC-HRMS, liquid-chromatography high-resolution mass spectrometry